Products specifications
|
Antigen
|
Enolase, Neuron Specific (NSE)
|
|
Reactivity
|
Human
|
|
Host
|
Mouse
|
|
NCBI Accession
|
RPA537Hu22
|
|
Concentration
|
500ug/ml
|
|
Conjugate
|
No Conjugate
|
|
Immunogen
|
Recombinant NSE (Met1~Leu434) expressed in E.coli.
|
|
Immunoglobulin Type
|
IgG
|
|
Purification
|
Affinity Chromatography
|
|
Specificity
|
The antibody is a mouse monoclonal antibody raised against NSE. It has been selected for its ability to recognize NSE in immunohistochemical staining and western blotting.
|
|
Application
|
WB, ICC, IHC-P, IHC-F, ELISA
|
|
Amino Acid Sequence
|
MGHHHHHHSGSEF-MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
|
|
Shipping Conditions
|
2 °C - 8 °C
|
|
Storage Temperature
|
Store at 4 °C for frequent use. Store at -20 °C to -80 °C for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
|
|
Handling Instruction
|
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
|
|
Notes
|
All antibodies are custom made on-demand. Please allow 5-7 working days for production and 2-3 days for shipment.
|
|
Legal Information
|
Marketed under license from USCN Life Science Inc.
|
Optimal working dilutions must be determined by end user.
| Western blotting | 1:100-400 |
| Immunocytochemistry in formalin fixed cells | 1:100-500 |
| Immunohistochemistry in formalin fixed frozen section | 1:100-500 |
| Immunohistochemistry in paraffin section | 1:50-200 |
| Enzyme-linked Immunosorbent Assay | 1:100-200 |